The precise identification of microbial strains that produce enzymes of industrial importance, such as pectinase, is crucial for specific biotechnological uses. This study highlights the function of pectinase in many industrial processes, adding to the expanding area of microbial enzyme technology. In this research, effective bacterial isolates that produce pectinase were characterized at the molecular level using 16S rRNA gene sequencing. Genomic DNA was extracted from chosen isolates derived from decomposed fruit and vegetable materials, which was then amplified through PCR and analyzed with agarose gel electrophoresis. The sequencing of the amplified products and subsequent BLAST analysis indicated that the isolates S1C1 and S3C3 were identified as Priestia flexa (previously known as Bacillus flexus) and Bacillus subtilis, respectively, exhibiting more than 98% sequence similarity. These results not only validate the taxonomic identity of high-performing strains identified in pectinolytic assays but also emphasize the importance of molecular tools in microbial classification and enzyme biotechnology. Pectinase enzymes are essential for a number of industrial processes, such as waste management, textile manufacturing, and food processing. The results indicate possible uses in commercial enterprises and emphasize the importance of microbial sources in sustainable enzyme synthesis.

Pectinase, Microbial enzyme technology, PCR, BLAST Analysis, Industrial Biotechnology, Agarose gelelectrophoresis, Sustainable enzyme synthesis