Micro sampling methods have really changed the game in bioanalysis. They let us grab tiny, exact amounts of body fluids with hardly any discomfort, making it all about the patient and saving resources Dried blood spots (DBS), volumetric absorptive micro sampling (VAMS), and Mitra devices are now key methods because they’re easy to use, stable, and work well with high-tech analysis. These methods cut down on the downsides of regular blood draws, like needing lots of blood, complicated storage, and moving it around DBS has been a game-changer for newborn screenings, tracking infections, and studying how drugs move through the body, but it’s got some issues with blood cell counts and making sure the samples are consistent VAMS and Mitra devices make it easy to get consistent, accurate samples, cutting down on differences caused by hematocrit levels and spotting. These new improvements are boosting efficiency and precision, opening up more possibilities for using drugs in clinical settings, checking for toxins, and running trials from different locations. The COVID-19 pandemic really showed us how much we need DBS, VAMS, and Mitra gadgets for people to do their health checks and keep an eye on their health from home, especially when we can’t move around much Even though there are hurdles such as keeping the analytes stable, making sure the process is consistent, and filling in the gaps in regulations, these issues are slowing down how widely clinical use is accepted. This review takes a deep dive into their technical principles, bioanalytical performance, applications, and key challenges, highlighting the innovations and regulatory strategies that are crucial for tapping into the full potential of micro sampling in personalized medicine, epidemiology, and ethical research.

Micro sampling, Dried Blood Spots (DBS), volumetric absorptive micro sampling (VAMS), Bioanalysis, Mitra devices, Therapeutic drug monitoring