Background: In adults as young, sclerosis as multiple considered as most cause common of disability as neurologic. MS is characterized by demyelination, inflammation, and injury of neuro-axonal at nervous central system causing longterm developing disability. At the current time no definitive single exam for MS diagnosis and monitoring. Objective: The aim of present study is to find out a non-invasive diagnostic neurofilaments biomarker (Neurofilaments light chain, Phosphorylated Neurofilament, Heavy Polypeptide) (NFL-L and P-NFL-H) for multiple sclerosis with high sensitivity and specificity. Patients and methods: A case control study based on three groups. first group 48 patients with previously diagnosis as MS and under treatment which include (11 male and 39 female), also second group was 20 patients new cases without treatment or who have sign of MS or suspected MS which include 7 male and 13 female who were observation in Middle-Euphrates-Neuroscience-Center in Anajaf AlAshraf/ Iraq. Third group was include 20 healthy volunteers (non-MS).

Health, Neurosystems, Circulating Neurofilaments, Diagnostic.