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Home > Archives > Volume 20, No 15 (2022) > Article

DOI: 10.14704/NQ.2022.20.15.NQ88001

Fabrication, characterization and Evaluation of protective action of Fluoxetine HCl nanoemulsion for PC12 cells from cell death induced by Corticosterone

Ela Khatri, Shailja, Pawan Jalwal, Akhil Sharma


The objective of present study was to create, characterise, and assess Fluoxetine HCl nanoemulsion (FXH-NE) for PC12 cell protection against corticosterone-induced cell death. The FXH nanoemulsion was initially created using the water titration method, improved upon by BBD, and assessed. After conducting optimization research, an optimised batch was created, and its parameters were then tested in vitro and in vivo. The optimized nanoemulsion formulation (ONE) was clear, pH-1.2, viscosity-140 cP, droplet size-90 nm and zeta potential (-27.3 mV). In- vitro permeation study showed that about 94.866±0.039% &95.653±0.051 % drug was permeated in 0.1 N HCl&SGF, respectively. In the P12 cell line, the antidepressant action was seen. The information gathered indicated that a damage concentration of 400 µM corticosterone was suitable. Additionally, statistical analysis showed that cells treated for 48 hours with a 50 µmol/L FXH nanoemulsion and then subjected to a corticosterone shock had considerably higher vitality than untreated PC12 cells subjected to the same insult (F = 0.38, df = 2,21, p <0.001).The model group's PC12 cells' survival rate considerably dropped as compared to the control group, and the FXH nanoemulsion at doses of 50 (p < 0.01), 100 (p < 0.01), 200 (p < 0.01), and 400 μg/ml (p < 0.01) could reverse the decrease in cell survival rate induced by corticosterone.


Fluoxetine HCl,Nanoemulsion, PC12 cells, Corticosterone, Fluoxetine HCl nanoemulsion

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