DOI: 10.14704/nq.2022.20.11.NQ66342

Methanolic extract and crude sapogenin glycosidic mixture obtained from Dragea volubilis ameliorates scopolamine-induced memory impairment in mice.

Amit Sharma, Naresh Gill

Abstract

Background: Alzheimer’s disease (AD) is a major challenge for neurobiology. The aging population, particularly those over 65 years, is at a greater risk of dementia due to AD. Dragea volubilis abundantly found in south India has been extensively used as an ayurvedic medicine to treat many ailments. Chemical exploration yielded potential leads for the treatment of many diseases. Objective: The present study aimed to explore the cognition enhancement potential and neuroprotective properties of dragea volubilis extracts and a crude sapogenin glycosidic mixture obtained after preliminary studies. Materials and methods: Methanolic extract was prepared in the Soxhlet apparatus. The methanolic extract was further fractionated using a butanol and water mixture to obtain a crude sapogenin glycosidic mixture. Behavioral parameters were assessed through passive avoidance and Morris water maze in scopolamine-administered mice by dividing animals into 6 groups n=6. The brain TBARS, GSH, catalase, and anticholinesterase assays were done. Results: Methanolic extract (MDV) and crude sapogenin glycosidic mixture (AF) results indicated that Passive avoidance (SDL) and escape latency (EL) were significantly P<0.01, P<0.001 better as compared to normal saline and scopolamine in young and old mice. ACHE and TBARS levels were decreased significantly by P<0.01, P<0.001, and catalase and GSH levels were increased significantly by P<0.001 as compared to scopolamine. Conclusion: - Methanolic extract and crude sapogenin glycosidic mixture showed cognition improvement in mice along with donepezil as compared to normal saline and scopolamine. The results probably indicated its potential as a nootropic owing to its neuroprotective, anti-cholinesterase, and antioxidant properties.

Keywords

MDV, AF, crude sapogenin glycosidic mixture Dragea volubilis, AD

Full Text

References