DOI: 10.14704/NQ.2022.20.11.NQ66294

Callus Induction and determination of Secoisolariciresinol Diglucoside (SDG) in Flax (Linum usitatissimum L.) plant

Mohammed K. Jalal, B. Z. Kasab Bashi, Ayad Tala't Shaker

Abstract

Flax/Katan (Linum usitatissimum L.) is a valuable medicinal plant that produces polyphenols known as lignans, which are beneficial biologically active compounds with numerous health benefits. Because of their antioxidant capabilities, which aid in the reduction of oxidative stress. Secoisolariciresinol Diglucoside (SDG) is one of the main Lignans with anticancer, antibacterial, antiviral, and anti-inflammatory properties. On Murashige and Skoog (MS) basal media supplemented with various combinations and concentrations of NAA and BA, callus induction from Flax (Linum usitatissimum L.) cotyledon explants was investigated. A high-performance liquid chromatographic (HPLC) technique was used to measure the quantity of secoisolariciresinol diglucoside (SDG) in Flax callus culture. Results shows that maximum callus weight and protein content were obtained from cotyledon explants grown on MS media supplemented with 0.25 mg/L NAA and 0.4 mg/L BA. After 4 weeks of culture, the greatest fresh weight of callus was 20.9 g/jar, with the highest percentage of callus induction 80% and this treatment gave the highest SDG content 34.26 mg.g-1.

Keywords

flax; lignans; cotyledon; callus; SDG; NAA; BA; HPLC

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