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Home > Archives > Volume 20, No 11 (2022) > Article

DOI: 10.14704/NQ.2022.20.11.NQ66284

Effect of salt stress on medicinal plant fenugreek callus on the activity of None enzymatic antioxidants Malandialdehyde, Glutathione, and Ascorbic acid

Hala J. Asree, Siham A. Salim

Abstract

The research was conducted to study the effect of salt stress on the activity of None enzymatic antioxidants Malan di aldehyde (MDA) , Glutathione (GSH), and Ascorbic acid (AsA) in the callus of the fenugreek medicinal plant. About 250 mg of seeds' induced callus was subculture in MS medium including 1.0 mg.L-1 of both BA and NAA supplemented with four concentrations of NaCl salt (6, 9, 12, and 15 ds.m-1) in addition to the control treatment without salt (2.23 dS.m-1 ). Cultures were incubated in the growth room for six weeks at room temperature of 25 ± 2 °C and light intensity of 1000 lux for 16 hours, followed by 8 hours of darkness per day. The results showed high levels of Malandialdehyde (MDA) , with increased activity of Glutathione (GSH), Ascorbic acid (AsA) none enzymatic antioxidant as a natural mechanism of apparent resistance salt stress and the resulting increase in free radicals.

Keywords

Fenugreek, medicinal plant ,salinity, callus culture, none enzymatic antioxidant

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