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Home > Archives > Volume 20, No 11 (2022) > Article

DOI: 10.14704/nq.2022.20.11.NQ66155

Enzyme Assay Guided Isolation of Alpha Amylase Inhibitor from Manilkara hexandra Leaves

PATEL EKTA D.*, PATEL DIVYAKANT A., PATEL YOGESH K., SHAH DHWANI A., VEGAD KUNJAL L., PATEL N. J.

Abstract

There has been a conspicuous resurgence of interest in the use of newer approach to treat diabetes and its complication has been immerging out because of the limitation of recently available drugs. The selection of plant was carried out on the bases of invitro screening of four antidiabetic plant for α- amylase activity. Methanolic extract of Manilkara hexandra shows good alpha amylase inhibition activity compare to other plants studied. Manilkara hexandra leaves traditionally known as Khirni or Rayan are considered useful for the treatment of diabetes in previous study.The alpha amylase inhibitor was separated by sequential extraction using increasing polarity of solvent such as n-Hexane, Chloroform, Ethyl acetate, n-Butanol and bioassay guided TLC fractionation. The ethyl acetate subfraction 3 shows highest inhibitory activity was further analyze by phytochemical screening, TLC, UV, Mass, IR and 1HNMRspectroscopy. Spectroscopic and chromatographic data reveals that the ethyl acetate subfraction 3 is flavonoid quercetin like compound. The enzyme assay guided fractionation of methanolic extract results in isolation of quercetin from Manilkara hexandra leaves as an active α-amylase inhibitor.

Keywords

Flavonoid, Diabetes mellitus, Quercetin, Acarbose, α- Amylase assay

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