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Home > Archives > Volume 20, No 11 (2022) > Article

DOI: 10.14704/nq.2022.20.11.NQ66122

Different Detection Methods of Cryptosporidiosis

Basma Hosny Abd El hameed Ahmed, Reda lamei El gamal, Ghada Mahmoud Fathy, Asmaa M. Farouk al-Ghandour


Background: Cryptosporidium is a coccidian protozoan parasite infecting humans and animals worldwide. It was so named because of the absence of sporocysts within the oocysts (Hidden sporozoites). The first Cryptosporidium spp. was described by Tyzzer in 1907 in lab mice gastric mucosa and was named Cryptosporidium muris (C. muris). A few years later, another smaller species was identified in the small intestine of mice and named C. parvum. It is now recognized that Cryptosporidium causes self-limiting diarrhea in immunocompetent case, but chronic life threatening diarrhea in immunocompromised individuals and it was probably been a human pathogen since the beginning of humanity. The diagnosis of cryptosporidiosis is essential for proper interventions and proper management of patients. Environmental samples are required for source tracking, risk factors assessment, and detection of outbreaks. Most of the laboratories may not look for Cryptosporidium unless specifically asked for, and so some selection criteria can be used to look for Cryptosporidium such as immunocompromised individuals, children <5 years, farm visitors, travelers from non-endemic to endemic countries, and local outbreaks. Stool sample is the most commonly examined sample in the diagnosis of cryptosporidiosis, while sometimes small bowel aspirates, biopsies or tissue samples may be available: Cryptosporidium can be diagnosed by a number of techniques including: 1- Microscopic examination either by the wet mount preparation or stained smears with modified acid-fast stain or by fluorescent stains. 2- Immunological methods detecting either antigen or antibody.3- Histological examination of tissue biopsy. 4- Molecular methods for detection of parasite DNA


Cryptosporidiosis, Diagnosis

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