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Home > Archives > Volume 20, No 8 (2022) > Article

DOI: 10.14704/nq.2022.20.8.NQ44941

Optimization of Zinc Oxide Nanoparticles Biosynthesis from Morin Hydrate Using Box– Behnken Design for Enhanced Antioxidant and Antimicrobial Activity

Adeep Kujur, Sanjay J. Daharwal


In the present study, synthesis of zinc oxide nanoparticles (ZnONPs) through Morin Hydrate by a novel eco-friendly, rapid and easy biological method was investigated. Morin Hydrate is recognized as a major constituent of many herbs and fruits like Psidium Guajava (Indian guava), almond (Prunusdulcis), fig (Chlorophora Tinctoria). The methanolic stock solution of Morin Hydrate was prepared and employed as a reducing and capping agent to synthesize stable zinc oxide nanoparticles via biological reduction approach. The method was systematically optimized using response surface methodology based BoxBehnken design (BBD), considering the effect of various independent variables (factors) such as zinc nitrate [Zn(NO3)2] concentration, incubation time and temperature on the responses like particle size and zeta potential of synthesized zinc oxide nanoparticles. The optimum conditions were [Zn(NO3)2] (3mM), incubation time (3 hrs) and temperature (70°C). Morin Hydrate methanolic stock solution can reduces zinc ions (Zn ++) into zinc (Zn0 ) nanoparticles (ZnONPs) within 3hrs after heating the reaction mixture (70°C) as indicated by the appeared deep yellow color. The UV-Vis spectrum of bio synthesized and optimized ZnONPs shows a characteristic strong absorption peak of Surface Plasmon Resonance (SPR) at 380 nm. Fourier transform infrared (FTIR) spectroscopy affirmed the role of Morin Hydrate as a reducing and capping agent of zinc ions. ZnONPs were subjected to X-ray diffraction (XRD) analysis, which exhibits their crystalline nature. Scanning electron microscopy (SEM) showed spherical shape, confirming the presence of elemental zinc with particle size of around 100 nm. The average particle size (z-average) was found to be 90.1 nm, its polydispersity index was 0.476 and Zeta values were measured and it was found to be -37.8 mV with the peak area of 100% intensity. This indicates that the formed zinc oxide nanoparticles (MHZnONPs) are stable. MHZnONPs were then studied for antioxidant activities by DPPH assay method, which revealed significant antioxidant activity as compared to standard antioxidant ascorbic acid. Moreover, antimicrobial potential of ZnONPs against Staphylococcus aureus and Bacillus subtilis, showed maximum zone of inhibition of 14mm, at 10 µg/mL concentration. Finally from the findings, we concluded that the synthesized ZnONPs shows excellent antioxidant and antimicrobial activity.


Green synthesis, Zinc Oxide Nanoparticles, Response surface methodology, Box-Behnken Design, Antioxidant, Antimicrobial.

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