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Home > Archives > Volume 20, No 11 (2022) > Article

DOI: 10.14704/nq.2022.20.11.NQ66006

Docking Studies on the C-AMP – Dependent Protein Kinase A (CPKA) Of Fungus MagnaportheOryzae the Causative Agent of Rice Blast Disease in Oryza Sativa.

Tapas Ranjan Samal, M Jagannath, K Umadevi

Abstract

The fungus Magnaportheoryzae (Phylum: Ascomycota; order: Magnoporthales; family: Magnaporthaceae) is the causative agent of rice blast disease in Oryza sativa. The infection is initiated by a structure called appressorium produced by the mycelia of M. oryzae in its incubation periods. The earlier studies indicates that the enzymes Adenylate cyclase (MAC1) and catalytic sub unit of CAMP dependent protein kinase A (CPKA) were very essential for development of appressorium and parthenogenesis. So these proteins can be considered as target proteins for the development of drug to control the rice blast disease. In this study, docking analysis is carried out to investigate the atomic interaction and molecular mechanism between seven chemical compounds namely Balanol, Genistein, H8, H88, H89, Isoquinoline and Staurosporine and CPKA protein of Magnaportheoryzae. It was undertaken in three different components. a. Modeling of CPKA protein structure using phyre 2 server, Energy minimization and protein structure refinement by ModRefiner algorithm i.e., finding the molecular interaction between CPKA protein of M. oryzae and different ligands of the above said seven chemical compounds, using AutoDock 4.2 tool.

Keywords

Ankylosing Spondylitis, studies, docking

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