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Home > Archives > Volume 20, No 8 (2022) > Article

DOI: 10.14704/nq.2022.20.8.NQ44879

Phenotypic and Genotypic Detection of Some Virulence Factors in Enterococcus faecalisIsolated From Urine Specimens.

Nazar Adnan A. Al-turfi, Ahmed A. Hussein

Abstract

Background: Enterococci are responsible for a large proportion of Gram-positive UTI worldwide. Virulence factors and biofilm formation plays a crucial role in the pathogenesis of enterococcal infections, which also prevents antimicrobial agents from penetration. This research aimed to determine the incidence of UTIs, and detection of some virulence factors among Enterococcus faecalisisolates. Materials and methods:TwentyE. faecalis isolates from patients with urinary tract infection were identified; phenotypic and genotypic characteristics were investigated in Al-Najaf City, Iraq. Results:TheE. faecalis isolates showed varying presences of virulence factors. Phenotypically, biofilm formation was exanimated, all of these isolates exhibited their ability to biofilm formation.In addition, it is ability to produce Gelatinase, protease and hemolysin in 30%, 55% and 85% of the isolates, respectively. Virulence factors genes esp, gelE and hyl were found in 60%, 80%, 1% of isolates, respectively. Conclusion :E. faecalis may be have many virulence factors such as production of gelatinase, protease and haemolysin. In addition, to biofilm formation that exhibited by all isolated E. faecalis.Data of PCR techniques confirmed the ability of E. faecalis isolates to carry virulence factors genes such as (esp, gelE and hyl).

Keywords

Enterococcus faecalis, Virulence factors, Urinary tract infection, Biofilm formation.

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