DOI: 10.14704/NQ.2022.20.11.NQ66039

ANTIBIOGRAM OF METALLO-BETA- LACTAMASE AND EXTENDED SPECTRUM BETA-LACTAMASE PRODUCING PSEUDOMONAS AERUGINOSA FROM ICU PATIENTS.

Satyajeet K. Pawar, Shweta S. Patil, Pooja P. Chavan, G. S. Karande

Abstract

INTRODUCTION: Pseudomonas aeruginosa, is one of the important pathogens responsible for infection in hospitalized patients. P. aeruginosa is considered stubborn pathogens in any hospitalized setup as it is adaptable to multiple drug-resistant mechanisms and capacity to remain alive in most environments. Therefore, it is important to have prior knowledge of susceptibility patterns in particular area, as it becomes easy to choose appropriate antimicrobial against these resistant strains. AIM: To detect antimicrobial susceptibility pattern of extended spectrum beta-lactamase and metallo- betalactamase producing P. aeruginosa from ICU patients. MATERIAL METHODS: P. aeruginosa were isolated from various clinical specimens by standard methods. Antibiogram was obtained by Kirby–Bauer disc diffusion methods. Combined Disc Diffusion technique was used for detection of metallo- beta-lactamase and extended spectrum beta-lactamase producing P. aeruginosa RESULT: Prevalence of metallo-beta-lactamase and extended spectrum beta-lactamase producing P.aeruginosa was 46.25% and 33.75% respectively. Aminoglycosides and antipseudomonal penicillins like piperacillin and combination drugs like amoxicillin and clavulanic acid were the most sensitive antimicrobials against them. CONCLUSION: The study underlines the unique problem of P. aeruginosa infections in ICU setup. Also, it is very important to have routine surveillance studies for drug resistance mechanism by phenotypic detection method, especially for P. aeruginosa isolated from ICU.

Keywords

MBL, ESBL, Imipenem-EDTA test, Antibiogram, Susceptibility

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