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Home > Archives > Volume 20, No 8 (2022) > Article

DOI: 10.14704/nq.2022.20.8.NQ44784

Correlation between in vitro preparation techniques and asthenozoospermic semen with bacteriospermia on intra-uterine insemination outcomes

Reem Qahtan Abdullah, Saad Salih Al-Dujaily, Rehab Shafiq Al-Maliki


Background:The sperm preparation techniques used for intrauterine insemination (IUI) and other reproductive techniques are heavily based on the sperm parameters and their survival. Furthermore, sperm preparation methods allow for the removal of immotile sperm, leukocytes, and immature germ cells from the inseminated volume. Objectives:This study has been aimed to evaluate the effectiveness of in vitro preparation technique(s) for the elimination of bacteria from semen samples that will be used for IUI. Subjects, Materials and Methods:Fifty one patients were enrolled for intra –uterine insemination who attended Infertility Clinics at the High Institute for Infertility Diagnosis and Assisted Reproductive Technologies, Al-Nahrain University during the period from November, 2021 till the end of May, 2022.The semen samples from husbands were divided into normozoospermia,and asthenozoospermia following semen fluid analysis according to (WHO ,2021 &2010). The semen samples were cultured under specific conditions using different type of media including (blood, MacConkey, chocolate and mannitol agar), to identify bacterial growth before and after in vitro preparation and activation using simple washing and direct swim up techniques for IUI . Results: There was a highly significant different in progressive motile sperm (p=0.001) after in vitro sperm activation compared to before activation in infected normozoospermic and asthenozoospermic infertile men growth of pathogenic bacteria. The main bacterial growth recoded in this study was Streptococcus feacalis , Klebsiella, Staphylococcus aureus ,E coli. Two out of 51 couples were pregnant following IUI one of them from normozoospermic man (10.0%) and the other from asthenozoospermic man (5.5%) with total 3.9% pregnancy rate. Conclusions: From practical point of view, Both preparation techniques prior to IUI leads to significant increases in certain sperm function parameters with significant bacterial reduction in most semen samples but not all semen bacterial clearance was obtained. The study recommended to culturing all semen samples not responded to the treatment


Asthenozoospermia, IUI, in vitro preparation techniques , Bacterial growth.

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