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Home > Archives > Volume 20, No 8 (2022) > Article

DOI: 10.14704/nq.2022.20.8.NQ44772

Screening of fractions of endophytes of Catharanthusroseusagainst HT 29 induced Colon Cancer in Rats

Sunil Kumar Kadiri, Rasapelly Ramesh Kumar, Samaresh Pal Roy, RakamGopiKrishna, Ponnala Soumya


Malignanttumor cells expected to grow rapid, and chemo medications kill fast developing cells. Since these medications travel all through the body, they can influence normal, healthy cells that are fast developing as well. Harm to healthy cells causes adverse effects. Avariety of bioactive molecules, including anti-neoplastic agents, have been recognized from various fungi. The aim of this study is to explore theanticancer activity of fungal endophytesproduced by Catharanthusroseus in HT 29 colon cancer cells in rats.An anticancer activity of portions of endophytes of Catharanthusroseus was assessed in HT 29 (Human colorectal adenocarcinoma cell line)inducedcolon cancer in rats. Cell line was presented intraperitoneally and following 24 hours, treatment was given for 14 continuous days. After anticancer therapy, blood was procured and hematological variables like RBC, WBC and hemoglobin were assessed and colons were taken apart for histopathologicalinvestigations and aberrant crypt foci (ACF) assay was conducted.From ACF test result, % inhibition in ACF was determined for all rats and all the data obtained were investigated statistically.Treatment of chloroform extract of Mucor sp. remarkably displayed % inhibition in ACF, and normalized the hematological profiles contrasted with those of pathological control rodents. After finishing of treatment, rodents displayed huge increase in body weights.Chloroform extracts of endophytic fungi derived from Catharanthusroseus which was distinguished as Mucorsp. have significant free radical scavenging and anticancer action. This antioxidant action of endophytes might be answerable for their anticancer action and might be because of the secondary metabolites of fungus catharanthusroseus. This data upheld the case that endophytes are considered as an elective hotspot for new secondary metabolites


Endophytes,Catharanthusroseus, HT 29 cancer cells, Anticancer activity, Mucorsp antioxidant action, Secondary metabolites

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