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Home > Archives > Volume 20, No 8 (2022) > Article

DOI: 10.14704/nq.2022.20.8.NQ44770

EVALUATION OFPHENOLIC, FLAVONOID CONTENTAND ANTIOXIDANT ACTIVITYOF ASPIDOPTERYS CORDATA: AN INDIGENOUS INDIAN PLANT

P. Udaya Chandrika, K. Sunitha

Abstract

The swing towards herbal medicine is an amassing aspect in modern therapy to maintain social and community healthreferable toas its safety margin and manifoldassets. Polyphenolics such as tannins and flavonoids are evidenced as powerful antioxidants to resolve cellular stress,endure homeostasisin the body and avert stress-associated syndromes.Consistentconsumption of antioxidants is a substitute to avoid serious illness. The currentinvestigationaimed to measure the phenolic, flavonoid content and antioxidant activity of Aspidopterys cordata, an endemic plant to India.Total phenolic content was estimated by Folin–Ciocalteu colorimetric method taking gallic acid as standard. Whereas, total flavonoid content was determined by Aluminum chloride colorimetric assay usingQuercetin as standard. The absorbance was measured at 760 nm and 510 nm respectively. Antioxidant activity was measured by two Invitro methods (DPPH and NO free radical scavenging assay) using standard protocols, where Ascorbic acid served as a reference standard.From the results, it was observed that the methanolic extract of A. cordata(106.24±0.75)was opulent with phenols. Similarly, flavonoid content inmethanolic extract (88.17±1.35)was found higher than other extracts. In the DPPH assay, methanolic extractA. cordata(81.07%)wasmore effective to inhibit the free radicalswith IC50 values39.704µg/ml, higher doses werecomparable to standard ascorbic acid (86.05%, IC50=34.29µg/ml). Similarly, in the NO free radical scavenging assay, methanolic extract ofA. cordata(79.39%) stood best among all to scavenge the free radicals with IC50 values 46.53µg/ml. Whereas, ascorbic acid was found to inhibit 84.22% with IC50 values 39.08µg/ml.The inhabitancy of various secondary metabolites such as alkaloids, carbohydrates, phenols, steroids, terpenoids, glycosides, saponins, especiallytannins and flavonoids, either alone or in conjunction may account for the marked scavenging property.The extent of the phenolic compounds and flavonoids can allydirectly to the displayedresults.

Keywords

Aspidopteryscordata, Antioxidant, Total phenolic content, Totalflavonoid content, Free radical scavenging

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