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Home > Archives > Volume 20, No 8 (2022) > Article

DOI: 10.14704/nq.2022.20.8.NQ44755

Microscopic Examination of Urinary Sediments in Hematuria

Dr. Saveta Arora, Dr. S V Jagtap

Abstract

Background and Aim: Hematuria is indicative of a wide range of etiologies of varying pathogenic significance. The microscopic examination of urinary sediments for the assessment of morphology of red blood cells (RBCs) is an economic, non-invasive screening to differentiate glomerular (GH) from non-glomerular hematuria (NGH). Hence, this study was performedto observe if ‘dysmorphic RBCs’ studied at early stages using Wright’s stain (WS) and light microscopy (LM) can help in investigation of hematuria. Methods:Thisprospective, observational study included 500 patients of either sex, aged 2 - 88 years, clinically diagnosed with significant hematuria (more than 3 RBCs/high power field). A detailed history of patient and data regarding possible causes of GH and NGH was collected.Urine sampleswere examined by WS. The slides were observed byLMfor crystals, squamous and transitional cells, bacteria, yeasts, or micro- organisms.All urine specimens were also examined by dipstick test to record urinary proteins and sugar. Statistical analysis was performed by using R software (Version. 3.6.0). Results:Majority of patients were in the age group of >60years with male preponderance (Male: female=2.13:1). A significant correlation was observed betweenproteinuria andglomerular disease (P < 0.05). Casts were present in 72.62% of GH patients among which RBC casts were most common (54.38%). In 4.87%NGH patients the casts were present, among which WBC casts were most common (1.77%). The total sensitivity of WS and LM was 83.94% and 77.37%in GH, whereas 89.82% and 88.50% in all the subgroups of NGH, respectively. Conclusion:It can be concluded that Wright stain can be used as a diagnostic tool in hematuria.

Keywords

Erthrocytes, Hematuria, Microscopy, Pathology, Urinalysis

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