DOI: 10.14704/nq.2022.20.8.NQ44752

Regulatory T Cells (CD4, CD25, FOXP3) and Acute Myeloid Leukemia (AML)

Alhussein Al Araby Ahmed Almeshaal, Nahla Abd Elmoneim Hamed, Nabil Ahmed ElHalawani, Rania Shafik Swelem, Dalia Ahmed Mohammed Nafie

Abstract

Background: Acute myelogenous leukemia represents a group of clonal hematopoietic stem cell disorders that result from genetic alterations in normal hematopoietic stem cells.It has been demonstrated that CD4 , CD25 and FoxP3 positivity characterize Treg cells in the peripheral blood (PB) using multicolor flow cytometry. Recent study,suggest that T reg may play a role in the pathogenesis of acute myeloid leukemia and measurements of T reg frequency may have clinical value in the clinical outcome.Aim:to measure Treg cells(CD4,CD25,Foxp3+ve(in newly diagnosed acute myeloid leukemia patients(AML) and to correlate it with cytogenetic studies for the evaluation of clinical outcome.Patients and Methods: This study was conducted on patients with newly diagnosed de novo AML they included25 AML pt and another 25 adults healthy group. All patients included in our study were subjected to full history taking, complete clinical examination ,routine laboratory investigations including complete blood count and bone marrow aspiration .Diagnostic peripheral blood and bone marrow samples were analyzed. All samples were studied for cytochemical staining ,immunophenotyping and finally detection of Treg cells by flow cytometry. Treg frequency was higher in AML patients at diagnosis compared to healthy controls. . However. Patients who achieved CR after induction chemotherapy had lower Treg levels compared with patients that had persistent leukemia. In Cytogenetic analysis We have not detected any significant difference in the percentage of circulating Treg between the patients who have an normal karyotype and those with abnormal karyotypes at presentation.Conclusion: There was an increase frequency of CD4CD25 and foxp3 Treg cells in PB in patients with AML.Patients who achieved CR after induction chemotherapy had lower Treg levels compared with patients that had persistent leukemia.

Keywords

Regulatory T Cells, Acute Myeloid Leukemia

Full Text

References