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Home > Archives > Volume 20, No 8 (2022) > Article

DOI: 10.14704/nq.2022.20.8.NQ44694

Histological study of adding different concentrations of plantago lanceolata L in Rose (308) strain broiler chickens

Shaimaa Allawy, Kadhim saleh Kadhim


The aim of this research is to test the effect of adding 1 gm, 3 gm and 5 gm of plantagolanceolata powder per kg of basic diet food on the histological structure of liver and intestine. In this study, Two hundred (200) Rose (308) strain unsexed broilers were employed, and the experiment was run from day 1 of chicken life until five weeks later (35 day old). The broilers were divided into four groups, each of which contained fifty broilers. The first group (T1) received a basic diet; the second group received a basic diet plus 1 gm/kg of plantagolanceolata; the third group received a basic diet plus 3 gm/kg of plantagolanceolata powder; and the fourth, a basic diet plus 5 gm/kg of plantagolanceolata powder. The ambient temperature was 32°C during the first week of life and then dropped by 2°C per week until it reached 25°C at the end of the experiment. Regarding the light. One hour of darkness and 23 hours of light were supplied. Blood from the wing vein was obtained in a test tube at day 35 of age without the use of an anticoagulant. Serum was obtained by Centrifuging the blood for 5 minutes at 3000 rpm, and it was then placed in a deep freezer (-20) until analysis. After sacrifice the animal liver and intestine sample has been taken and they were immediately put into a 10 % fixative formalin solution. Through the current study the result show that there is a significant decrease in serum GOT in T4 group when compared to the other groups. Also Albumin showed a significant increase in T3 and T4 when compared to the other group. Histological result show liver showed mild central vein dilatation slightly dilated sinusoids separating hepatocytes lobules.


plantagolanceolata, broilers, Rose (308),liver enzyme, liver structure.

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