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Home > Archives > Volume 20, No 8 (2022) > Article

DOI: 10.14704/nq.2022.20.8.NQ44631

Optimization of important factors for efficient embryogenesis in sweet pepper (Capsicum annuumvar.grossumL.) using anther culture technique

Kaushik Jha, Pradeep Kumar Choudhary, Arun Agarwal

Abstract

Different combinations of factors affecting androgenic embryo induction in four sweet pepper hybrids, namely Asha, Sympathy, Namelite, and Indra were investigated in this present study. There were three combinations of different factors which were employed such as cold pre-treatment with heat shock, sucrose with activated charcoal, and auxin with cytokine. The experimental results revealed that the highest number of embryos (39.3) per 300 anthers were recorded in ‘Asha’ (V1) when buds were cold pre-treated at 4°C for 24 hours (C2) followed by keeping anthers at 35°C in the dark for 12 days +25o C-Cont. (HS3). The combination of sucrose with activated charcoal gave the highest number of embryos (48.7) in ‘Asha’ (V1), when anthers were excised and placed on CP induction media supplemented with 4% (S3) sucrose and 0.50 g/l (AC2) activated charcoal. The combination of 2,4-D with Kinetin revealed the highest number of embryos (43.0) in ‘Asha’ (V1)where anthers were excised and placed on media supplemented with 0.50 mg/l of 2,4-D (A2) with 4.0 mg/l of Kinetin (K4). An additional experiment was performed by combining the best treatments from the previous experiments, and the highest frequency of embryo induction (23.1%) was observed in ‘Asha’ (V1). The embryo formation was primarily observed to be genotypedependent, with Asha being the most responsive in every set of combinations studied, and Sympathy (V2) being the least responsive. Aside from genotype dependence, factors, such as cold pre-treatment, heat shock, activated charcoal, carbon source, and plant growth regulators play an important role in embryo induction.

Keywords

Capsicum annuumvar.grossum, anther culture, embryo induction, genotype effect.

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