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Home > Archives > Volume 20, No 8 (2022) > Article

DOI: 10.14704/nq.2022.20.8.NQ44556

ANTIOXIDANT AND ANTI-INFLAMMATORY PROPERTIES OF NYCTANTHES ARBOR-TRISTIS LEAF EXTRACTS IN HIGH GLUCOSE-INDUCED SPRAGUE DAWLEY RATS

Dharma Raj Tanimale, Geethaa Sahgal, Sherly Deborah George, Fong Lai Yen, Mohamed Asem, Pandian Paneer Selvam, Ng Chin Theng

Abstract

Objective: To determine the phytochemical constituents and antioxidant activity of N. arbor-tristis leaf extracts, and also to investigate the in vivo anti-inflammatory effect of N. arbor-tristis water extract in high glucose-induced Sprague Dawley rats. Methods:The ethanolic and water extracts of N. arbor-tristiswere prepared by soaking oven-dried powdered leaves in ethanol and distilled water, respectively.The antioxidant activity and total antioxidant capacity of the leaf extracts were assessed using DPPH scavenging method and phosphomolybdenum assay, respectively. The changes in the vascular permeability and leukocyte migration were assessed using an Evans blue assay. Results:The phytochemical studies exposed that water extract of N. arbor-tristis containsrelatively high amounts of flavonoids and saponins, compared to the ethanolic extract. N. arbor-tristis water extract also showed significantly higher DPPH radical scavenging activity and total antioxidant capacity than the ethanolic extract. In vivoresults showed that 1000 mg/kg and 2000 mg/kg of N. arbor-tristis water extract significantlyattenuated D-glucose-induced increased vascular permeability and leukocyte migration. The results advocate that the antioxidant and anti-inflammatory properties of N. arbortristismight be related to the high flavonoid and saponin contentsfound in the leaves. Conclusion:This study demonstrated that N. arbor-tristis water extract possesses antioxidant activity and a novel protective role against vascular inflammation in vivo. This reveals the rational use of N. arbor-tristis leaf extracts in traditional medicinal practice, and the possibility to further explore the therapeutic effect of N. arbor-tristisin vascular inflammatory diseases.

Keywords

Nycthanthesarbor-tristis, antioxidant, anti-inflammatory, high glucose

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