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Home > Archives > Volume 20, No 8 (2022) > Article

DOI: 10.14704/nq.2022.20.8.NQ44550

Evaluation of Bacteriological Quality of Treated Water and Dialysate in Haemodialysis Unit: A microbiological study

1Dr. Farkhanda, 2Dr.Shama Tomar, 3Dr.Pratik Tripathi


Background: The present study was conducted for evaluating Bacteriological Quality of Treated Water and Dialysate in Haemodialysis Unit. Materials & methods: Sample size for the present study was 225. Total number of 25 treated water samples and 200 Dialysate samples were analysedduring the time period of six months i.e from January 2022 to June 2022. Treated water samples were collected immediately past the water purification system (Reverse Osmosis) water tank and from Reverse Osmosis (RO) lines supplying the HD unit. From each haemodialysis machine, dialysate samples were obtained where dialysate exits the dialyzer in the HD unit. For treated water, the colony count/ml was determined by multiplying the number of colonies seen by 10. The number of colonies seen was multiplied by 1000 to get the colony count per ml of dialysate sample. The acceptable limits of bacterial contamination for Treated water and Dialysate were taken as less than 200 CFU/ml and less than 2000 CFU/ml respectively. All the results were recorded in Microsoft excel sheet and were analysed by SPSS software. Results: 173 out of 200 dialysate sample showed no positive for bacterial colony forming unity. 27 out of 200 dialysate sample showed positive growth for bacterial colony forming unit which were less than 50cfu/ml. 18 out of 25 treated water samples showed-negative growth. 7 out of 25 treated water samples showed positive growth for CFU. Conclusion: Quality of dialysis water is dependent upon quantity of microbial contamination of untreated water, water purification techniques used and maintenance of water treatment and distribution systems.


Haemodialysis, Dialysate, Bacteriological

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