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Home > Archives > Volume 20, No 8 (2022) > Article

DOI: 10.14704/nq.2022.20.8.NQ44527

Estimation of Diclofenac diethylamine and Plumbagin in a drug delivery system by simultaneous equation method

Ajita Khichariya, Gunjan Jeswani, Vinay Sagar Verma, Subhash Sahu, Shahbaz Rathor, Amit Alexander , Kartik T Nakhate, Hemant Ramchandra Badwaik

Abstract

Objective The aim of this study was to develop and validate UV-Vis spectrophotometric method for simultaneous estimation of Diclofenac diethylamine and Plumbagin in a combined dosage form. Method Solubility studies were performed to select solvent for UV-Vis spectrophotometric analysis. λmax values were selected on the basis of analysis of overlay spectra. Simultaneous equation for estimation of drugs (Diclofenac diethylamine and Plumbagin) in a combined dosage form was developed using value of absorbance and absorptivity. Key findings Methanol was selected as solvent on the basis of solubility study. Two λmax values, 282nm and 418nm were selected as wavelength of detection. Diclofenac diethylamine and Plumbagin both followed Beer's law over concentration ranges of 5 to 25 μg /ml and 5 to 40 μg /ml, respectively. Conclusion The simultaneous equation for estimation of Diclofenac diethylamine and Plumbagin was developed according to ICH guidelines and validated for linearity, accuracy, precision, robustness, limit of detection and limit of quantification

Keywords

UV-Vis Spectroscopy; Simultaneous Equation Method; Diclofenac diethyl amine; Plumbagin

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