DOI: 10.14704/nq.2022.20.8.NQ44489

Trigonella Foenum Grecum L. Seed (Fenugreek) Pharmacological Effects on Cardiovascular and Stress Associated Disease

Arpan Kumar Tripathi, Priya Diwedi, Narendra Kumar, Bhupendra Kumar Yadav, Dewanjali Rathod

Abstract

The major goal of this study is to determine the antioxidant activity of Trigonella foenumgrecum L. seeds. The importance of the bioactive chemicals discovered by GC-MS in these seeds. Standard methods for determining antioxidant activity included the 2,2-diphenyl1-picryl-hydrazyl (DPPH), Superoxide dismutase (SOD), and Catalase (CAT) tests. By producing a graph of percent inhibition versus concentration, the concentration of the sample necessary to scavenge 50% of free radicals was estimated2.8741 and 3.8372 were reported to be the IC50 values for 2,2-diphenyl 1-picrylhydrazyl(DPPH) radical activity in aqueous and methanolic extracts, respectively. The number is almost identical to the ascorbic acid control value of 2.5391. For aqueous extract, the percent inhibition and specific activity of Catalase (CAT) were found to be 77.38 percent and 34.227 µmoles of Hydrogen peroxide~H2O2/min/ml, respectively. For methanolic extract, the percent inhibition and specific activity of Catalase (CAT) were found to be 82.42 percent and 26.0153µmoles of H2O2/min/ml, respectively. For aqueous extract, the percent inhibition and specific activity of SOD were found to be 53.21 percent and 1.5843 units/mg, respectively. For methanolic extract, the percent inhibition and specific activity of Superoxidedismutase (SOD) were found to be 61.24 percent and 1.3848 units/mg, respectively. This indicates that the methanolic extract from Trigonella foenum grecum L seeds showed greater antioxidant activity than the aqueous extract. The findings of this in-vitro study indicated that this species of Trigonella foenum grecum L is suitable for use as a raw medication and is the best treatment for cardiovascular disease (CVD). In-vivo investigations may be conducted in the future to determine the antioxidant capability of these species' seeds in a living system.

Keywords

Apigenin,Antioxidant, Flavonoids, Trigonellafoenumgrecum, methi

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