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Home > Archives > Volume 20, No 8 (2022) > Article

DOI: 10.14704/nq.2022.20.8.NQ44446

Molecular Diagnosis and Genetic Polymorphism of Entamoeba Histolytica in Iraqi Samples

EnasTaha Mossa1*, Dr. Habeeb Wasel Shubber2

Abstract

The aim of this study was to determine the prevalence of the E. histolytica between males and females, as well as to determine its spread depending on the type of infection, whether it is associated with bacteria or not, in addition to studying the E. histolytica genetically through RNA to determine the genetic sequence of this E. histolytica A total of 100 samples (stool) were collected from patients suffering from diarrhea with inflammatory feature from several different locations in the Adiwaniyah City, Iraq, including: Al-Diwaniya Teaching Hospital, Maternityand Children Hospital, Hamza general Hospital and some many private medical laboratory during the period from october 2021 to April 2022. The current study was done at AL-Qadisiyah University. The stool samples were classified to be healthy, infected with E.histolytica according to genetic examinations. The results showed E.histolytica and bacteria infection higher in malethan female; it were in male30(60%) while lower results in entamoeba only 8(16%). There aresignificant differencesamongstthree groups.In the female high results show mixedinfection of E.histolytica and bacterial infection which recorded 18(36%)and parasite infection only recorded the same results,while bacterialinfection only showed that 4(8%). The current study concluded that the mentioned parasite affects males more than females, as well as infection of the type associated with bacteria is the most prevalent in males and females, and the current study concluded that there are mutations that occurred in the parasite’s genome that can increase the virulence of this parasite.

Keywords

E. histolytica, Intestinal Infections,E.coli.

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