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Home > Archives > Volume 20, No 8 (2022) > Article

DOI: 10.14704/nq.2022.20.8.NQ44405

Extraction and Identification of Phytoconstituent obtained from Streblus as per Lour Root Bark as an Antibacterial Agent

Mrinmoy Nag, Rakesh Kumar Paul, Souvik Biswas, Dipan Roy, Rajdeep Saha,Poushali Boral, Souvik Chattopadhyay, Debajit Dewan

Abstract

Streblus asper Lour has been used in Ayurveda to treat microbial infections. The current research looks at the quantity of Lupeol in a Streblus asper root bark (SARB) extract, as well as its antibacterial activity and in silico studies. The SARB contain lupeol, which is having antibacterial activity. Time course assay, disc diffusion method, minimum inhibitory concentration (MICs) using broth dilution method, and pH sensitivity assay were used to assess the antibacterial activity of SARB extract against gram-positive and as well as gram-negative bacteria.In silico molecular docking studies, Lupeol has good binding activity against MurE E. coli than MurE S. aureus. E. coli was inhibited by the plant extract SARB at MIC ≥625 μg ml-1, whereas S. aureus was inhibited at MIC ≥1250 μg ml-1. The SARB demonstrated a remarkable MBC against the tested organism. In case of time response assay, suggested that plant extract completely inactivated E. coli and S. aureus within 6 hrs and 8 hrs respectively. The amount of lupeol present in SARB was found to be 4.63% (w/w) through RP-HPLC, which indicated significant amount of lupeol present in the extract. Our study suggests that Streblus asper can be useful as a potential natural antimicrobial agent.

Keywords

Streblus asper, Ayurveda, RP-HPLC, In silico, Antibacterial

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