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Home > Archives > Volume 20, No 7 (2022) > Article

DOI: 10.14704/nq.2022.20.7.NQ33075

Detection of Some Antibiotic Resistance Genes of Acenitobacter bumannii isolated from burn patients

Dr. Arman Rostamza, Nada Aziz maha

Abstract

Infections caused by Acinetobacter baumannii are a formidable threat because of its ability to survive on inanimate objects and the remarkable emergence of multi-drug resistant (MDR) and pan-drug resistant (PDR) strains. The aim of this study was to characterize the antibiotics resistance genes of A. baumannii isolated from clinical specimens, in order to investigated the immune response of IL 17 in patients toward bacteria. The current study was includes 100 clinical samples collected from patients suffering of burn ,who attended to medical burn center ,al Hakim general Hospital from December (2021) to February (2022). Out of (100) clinical samples were obtained only 50 isolates belonging to A. baumanniii that were detected through several laboratory tests, including morphological , biochemical test and the isolates of A. baumanniii confirm by PCR technique. The distribution of isolates were from Wound swab (25), Burn(15),and Blood (10) . The results revealed variation in bacterial resistance to antibiotics . A. baumanniii express high resistance against Ceftazidime, cefapime , Meropenem, cefoxitin and Tobramycin (100%, 91% ,88%, 86% and 83%) respectively , while other isolates revealed moderate resistance to antibiotic such as : Ciprofloxacin (48%) the weak resistance to antibiotic such as Doxycycline, and Imipenem (18% , 10%) respectively, The results showed that all isolates have been using the PCR technology for three of the important genes that encode the antibiotic resistance of the genes that encode the resistance of the bacteriostatic species (CTX-M-15, bla TEM, bla PER) .which was the result of the acquisition of isolates (50) of these genes (bla TEM, bla PER, CTX-M-15) are 80%, 68%, and 18%, respectively. The A. baumannii capable to high resistance the most antibiotic including Ceftazidime, cefapime , Meropenem, cefoxitin and Tobramycin but weak resistance to the imipenem only for the antibiotic used in this study . The A. baumnnii carry the genes that has resistance antibiotic including the carbapenemase , metalo -B- lactamase and extended spectrum – B-lactamase (ESBLs ) genes (of blaTEM, blaPER, blaCTX-M 15 .

Keywords

Antibiotic Resistance, PCR , A. baumannii , blaTEM, blaPER, blaCTX-M 15.

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